Species of the genus Gossypium have varying degrees of leaf hairiness. In the wild species G.tomentosum Nutt. ex Seem this feature has a unique twisted appearance. In backcrossed plants, interspecific hybrids involving this species have a high density of plant hairs, and spiral hairs from the wild species provide low pest infestation. In order to study heredity and preserve this type of pubescence in generations, our studies by crossing introgressive lines with analyzer lines found that the number of dominant alleles and auxiliary genes is of great importance in the inheritance of spiral pubescence. And the number of hairs and the thickness of the leaf are controlled by cytoplasmic genes. The number of hairs per 1 mm2 of the leaf was genetically analyzed, while the “spiral” type of pubescence in the offspring completely dominates, and in the heterozygous state it exhibits the “spiral” phenotype. By the nature of the pubescence in F2, the lines of the spiral type are allelic states of the same gene as the lines with the usual type of hairiness. By the nature of pubescence in F2, it was proved that lines with twisted hair type differ from lines with simple hair type by alleles of the same gene.