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POLYMERASE CHAIN REACTION (PCR)

Authors

To‘raboyeva Mohinur Dilmurodjon qizi Andijon davlat universiteti
Alimova Laylo Omadjon qizi Andijon davlat universiteti
Ne'matullayeva Mohidil Muqimjon qizi Andijon davlat universiteti
Tuxtaboyeva Feruzaxon Muratovna Andijon davlat universiteti

Abstract

This thesis describes in detail the importance of polymerase chain reaction (PCR) in molecular biology and medicine, its working principle and practical applications. The main stages of PCR - denaturation, annealing and elongation processes - are explained from a scientific point of view. Also, the functions of the components necessary for the successful course of the reaction - template DNA, primers, dNTPs, thermostable polymerase and buffer solutions are analyzed. The thesis compares and shows the advantages and limitations of various modifications such as classical PCR, Real-Time PCR (qPCR), RT-PCR, multiplex PCR. The role of PCR in clinical diagnostics, genetic research, forensics, biotechnology and ecology is illustrated with examples. This work deeply illuminates the role of PCR technology in modern scientific and practical activities due to its relevance, high sensitivity and accuracy.

References

Ghannam mg, varacallo ma. Statpearls [internet]. Statpearls publishing; treasure island (fl): jul 30, 2023. Biochemistry, polymerase chain reaction.

H. Karimov. “biotexnologiya asoslari”. – toshkent: tdpu o‘quv qo‘llanma.

Islam ku, iqbal j. An update on molecular diagnostics for covid-19. Front cell infect microbiol. 2020;

Lorenz tc. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. J vis exp. 2012 may 22

M. Xasanov, a. Jo‘rayev. “molekulyar biologiya”. Oliy ta’lim uchun darslik. – toshkent: o‘zbekiston nashriyoti.

Mackay im, arden ke, nitsche a. Real-time pcr in virology. Nucleic acids res. 2002 mar

Markham af. The polymerase chain reaction: a tool for molecular medicine. Bmj. 1993 feb 13

O‘zbekiston respublikasi otmlari uchun “mikrobiologiya va virusologiya” darsligi

R. Zokirov, sh. Tursunov. “genetika asoslari”. – toshkent: fan nashriyoti.

Ramesh r, munshi a, panda sk. Polymerase chain reaction. Natl med j india. 1992 may-jun;5

Ruijter jm, barnewall rj, marsh ib, szentirmay an, quinn jc, van houdt r, gunst qd, van den hoff mjb. Efficiency correction is required for accurate quantitative pcr analysis and reporting. Clin chem. 2021 jun