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POLIMERAZA ZANJIRI REAKSIYASI (PCR)

Mualliflar

To‘raboyeva Mohinur Dilmurodjon qizi Andijon davlat universiteti
Alimova Laylo Omadjon qizi Andijon davlat universiteti
Ne'matullayeva Mohidil Muqimjon qizi Andijon davlat universiteti
Tuxtaboyeva Feruzaxon Muratovna Andijon davlat universiteti

Abstrak

Ushbu tezis ishida polimeraza zanjiri reaksiyasi (PCR)ning molekulyar biologiya va tibbiyotdagi ahamiyati, ishlash prinsipi hamda amaliy qo‘llanilish sohalari batafsil yoritilgan. PCRning asosiy bosqichlari – denaturatsiya, annealing va elongatsiya jarayonlari ilmiy nuqtayi nazardan izohlangan. Shuningdek, reaksiyaning muvaffaqiyatli kechishi uchun zarur bo‘lgan komponentlar – shablon DNK, primerlar, dNTPlar, termostabil polimeraza va tampon eritmalarining vazifalari tahlil qilingan.Tezisda klassik PCR, Real-Time PCR (qPCR), RT-PCR, multiplex PCR kabi turli modifikatsiyalarning afzalliklari va cheklovlari solishtirilib ko‘rsatildi. PCRning klinik diagnostika, genetik tadqiqotlar, kriminalistika, biotexnologiya va ekologiyada tutgan o‘rni misollar bilan yoritildi.Mazkur ish PCR texnologiyasining dolzarbligi, yuqori sezgirligi va aniqligi sababli zamonaviy ilmiy-amaliy faoliyatda tutgan o‘rnini chuqur yoritadi.

References

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