DETERMINATION OF PHENOL COMPOUNDS IN RADISH EXTRACT BY THE USSX METHOD AND ITS BENEFICIAL PROPERTIES
Keywords:
radish extract, phenolic compounds, USSX method, antioxidant activity, flavonoids, phenolic acids, bioactive substances, free radicals, natural antioxidant, health benefits.Abstract
This article describes the use of high-performance liquid chromatography (HPLC) to determine phenolic compounds in radish (Raphanus sativus) plant extracts. During the study, phenolic compounds were isolated from radish extract samples and their qualitative and quantitative composition was analyzed. Based on the results obtained, it was determined that radish extract contains phenolic acids and flavonoids with antioxidant activity. The beneficial effects of these compounds on human health, in particular, their role in neutralizing free radicals, anti-inflammatory properties, and strengthening the immune system, were also discussed. The results of the study indicate the potential of using radish extract as a natural source of antioxidants in the pharmaceutical and food industries.
References
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178.
Crozier, A., Jaganath, I. B., & Clifford, M. N. (2009). Dietary phenolics: Chemistry, bioavailability and effects on health. Natural Product Reports, 26(8), 1001–1043.
G‘aniev, I. G. (2018). Dorivor o‘simliklar va ularning biologik faol moddalari. Toshkent: Fan va texnologiya nashriyoti.
Ignat, I., Volf, I., & Popa, V. I. (2011). A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry, 126(4), 1821–1835.
Karimov, M. M. (2020). Fitokimyo asoslari. Toshkent: O‘zbekiston Milliy ensiklopediyasi davlat ilmiy nashriyoti.
Beecher, G. R. (2003). Overview of dietary flavonoids: Nomenclature, occurrence and intake. The Journal of Nutrition, 133(10), 3248S–3254S.
Raximov, S. R. (2017). Dorivor o‘simliklardan biologik faol moddalarni ajratib olish usullari. O‘zbekiston Kimyo jurnali, 3, 45–49.
Kim, S. J., Kawaharada, C., Suzuki, T., Sakamoto, M., & Kondo, N. (2006). Effect of growth stage on the content of glucosinolates and phenolic compounds in radish (Raphanus sativus L.). Journal of Agricultural and Food Chemistry, 54(11), 4067–4073.
Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects. Journal of Functional Foods, 18, 820–897.