QORAMOLLARNING TURLI ZOTLARIDA BETA-KAZEIN (CSN2) GENINI PZR USULIDA TAHLIL QILISH
Kalit so‘zlar:
Beta-kazein, CSN2, DNK, Polimeraza Zanjiri Reaksiyasi (PZR), elektroforez, Bushuyev, Simmental, Shvits.Abstrak
Ushbu ilmiy tadqiqot ishida qoramollarning turli zotlarida beta-kazein sinteziga javobgar CSN2 genining polimorfizmlarini turli qoramol zotlarida PZR (Polimeraza Zanjiri Reaksiyasi) usulida tahlil qilishning ahamiyati va metodologiyasi ko‘rib chiqilgan. Qoramollarning Bushuyev, Simmental, Shvits zotlaridan 20 tadan qon namunasi olindi va DNK ajratilib, CSN2 geniga PZR o‘tkazildi va 1,5% agaroza gelida PZR mahsulotlarning elektroforez tahlili amalga oshirildi. CSN2 genini amplifikatsiya mahsulotlarning elektroferogrammalarini tahlil qilish natijasida barcha namunada PZR mahsulotlari mavjudligi aniqlandi, PZR mahsulotlarining uzunligi 9000 j. n. tashkil qildi. Shunday qilib, CSN2 genida va uning promoter regionida insertsion-deletsion polimorfizmlar aniqlanmadi. CSN2 genida SNP polimorfizmlarini aniqlash uchun keyingi bosqichda PCR mahsulotlarining sekvenslash amalga oshirish zarur
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