SELECTION OF AN OPTIMAL SOURCE OF CARBON AND NITROGEN FOR BIOSYNTHESIS OF THE MAXIMUM AMOUNT OF PROTEASE

Abstract

In the present study, the effect of various carbon and nitrogen sources on protease biosynthesis by the Bacillus amyloliquefaciens strain UzRSMMT-413 was investigated. The results of the studies showed that the maximum protease activity was observed on the medium using 0.4% galactose as a carbon source and 2 g/l urea as a nitrogen source; the protease activity for this strain was 24 U/ml. It was shown that the strains exhibited the ability to synthesize protease in a wide pH range from 4.0 to 8.0 with an optimum of pH = 6.0; the enzyme was stable in the temperature range of 40-60 °C; when the temperature increased to 70 °C, the activity remained above 50% of the optimum, and only at 80 °C did it decrease to 30%.