PROTEAZA MAKSIMAL MAQDIRDAGI BIOSINTEZ UCHUN UGLEROD VA AZOTNING OPTIMAL MANBANI TANLASH.
Kalit so‘zlar:
Bacillus, skrining, proteaz, uglerod manbai, azot manbai.Abstrak
Ushbu tadqiqotda UzRSMMT-413 Bacillus amyloliquefaciens shtammi tomonidan turli xil uglerod va azot manbalarining proteaz biosinteziga ta'siri o'rganildi. Tadqiqotlar natijalari shuni ko'rsatdiki, maksimal proteaz faolligi uglerod manbai sifatida 0,4% galaktoza va azot manbai sifatida 2 g / l karbamiddan foydalangan holda muhitda kuzatilgan; bu shtamm uchun proteaz faolligi 24 U/ml edi. Shtammlar pH = 6,0 optimal bilan 4,0 dan 8,0 gacha bo'lgan keng pH diapazonida proteazni sintez qilish qobiliyatini namoyish etganligi ko'rsatildi; ferment 40-60 ° S harorat oralig'ida barqaror edi; harorat 70 ° C ga ko'tarilganda, faollik optimaldan 50% dan yuqori bo'lib qoldi va faqat 80 ° C da u 30% gacha pasaydi.
References
Tarek, H.; Nam, K.B.; Kim, Y.K.; Suchi, S.A.; Yoo, J.C., Biochemical Characterization and Application of a Detergent Stable, Antimicrobial and Antibiofilm Potential Protease from Bacillus siamensis. Int. J. Mol. Sci. 2023, 24, 5774. https://doi.org/10.3390/ijms24065774
Rachanamol, R.; Lipton, A.; Thankamani, V.; Sarika, A.; Selvin, J. Production of protease showing antibacterial activity by Bacillus subtilis VCDA associated with tropical marine sponge Callyspongia diffusa. J. Microb. Biochem. Technol 2017, 9, 270–277.
Hashmi, S.; Iqbal, S.; Ahmed, I.; Janjua, H.A. Production, Optimization, and Partial Purification of Alkali-Thermotolerant Proteases from Newly Isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12. Processes 2022, 10, 1050. https://doi.org/10.3390/ pr10061050
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