PREPARATION OF ALPHA AMYLASE-PRODUCING BACTERIA FOR PRODUCTION

Authors

  • Abduvohidov Ozodbek Abdurahmon o’g’li Andijon davlat universiteti
  • G'ulomov Saloxiddin Akmaljon o'g'li Andijon davlat universiteti
  • Xudoyberganov Xasanboy Muhammadumar o’g’li Andijon davlat universiteti
  • Nabiyev Qobiljon Qosimjon o’g’li Andijon davlat universiteti

Keywords:

Alpha-amylase; Bacillus; inoculum; submerged fermentation; solid-state fermentation; nutrient medium optimization.

Abstract

This article reviews the practical and scientific aspects of preparing alpha-amylase-producing bacteria for industrial production. Strain selection, isolation, inoculum preparation, media optimization, submerged and solid-state fermentation methods, and downstream purification steps are covered in detail. The possibilities of increasing productivity through genetic optimization, statistical-based environmental parameter tuning, and the use of agro-industrial waste are also discussed. The article provides recommendations for scientific and practical research and industrial projects.

References

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Hewitt, C. J., & Solomons, G. L. (1996). The production of α‑amylase (E.C.3.2.1.1.) by Bacillus amyloliquefaciens in a complex and synthetic culture medium. Journal of Industrial Microbiology and Biotechnology, 17(2), 96–99.

Zalila‑Kolsi, I., Ben‑Mahmoud, A., & Al‑Barazie, R. (2023). Bacillus amyloliquefaciens: Harnessing Its Potential for Industrial, Medical, and Agricultural Applications — A Comprehensive Review. Microorganisms, 11(9), 2215.

Ngalimat, M. S., Yahaya, R. S. R., Baharudin, M. M. A.‑a., Yaminudin, S. M., Karim, M., Ahmad, S. A., & Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9(3), 614.

Far et al. (2024). Optimization of the Alpha‑Amylase Production from Microbial Source: A Systematic Review of Experimental Studies.

Published

2025-12-12

Issue

Vol. 2 No. 12 (2025): UNIVERSAL INTERNATIONAL SCIENTIFIC JOURNAL

Section

Articles

License

Copyright (c) 2025 Abduvohidov Ozodbek Abdurahmon o’g’li, G'ulomov Saloxiddin Akmaljon o'g'li, Xudoyberganov Xasanboy Muhammadumar o’g’li, Nabiyev Qobiljon Qosimjon o’g’li

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

PREPARATION OF ALPHA AMYLASE-PRODUCING BACTERIA FOR PRODUCTION. (2025). Universal International Scientific Journal, 2(12), 170-173. https://universaljurnal.uz/index.php/jurnal/article/view/3817